ANKRD1 ELISA Kit (Mouse)

CAT: 0247-OKDD00781-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKDD00781-96WSize:96 Tests
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Reproducibility
Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Ankyrin repeat domain 1 (cardiac muscle)
Gene Aliases
Alrp; ankyrin repeat domain-containing protein 1; ankyrin-like repeat protein; C; cardiac ankyrin repeat protein; cardiac responsive adriamycin protein; CARP; Crap; MA; MARP1.
Gene ID
107765
Accession Number
NP_038496.2
Reactivity
Mouse|Mus musculus
Target
May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology ANKRD1 ELISA Kit (Mouse) (OKDD00781) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ANKRD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANKRD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ANKRD1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates
Detection Range
0.156-10ng/mL
Sensitivity
<0.052ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
36 kDa
Notes
Formerly GWB-KBBBD8
Specificity
This assay has high sensitivity and excellent specificity for detection of ANKRD1. No significant cross-reactivity or interference between ANKRD1 and analogues was observed.
Protein Length
319
NCBI Gene Symbol
Ankrd1
Protein Name
Ankyrin repeat domain-containing protein 1
Gene Name URL
Ankrd1
Nucleotide Accession Number
NM_013468.3

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