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MBL ELISA Kit (Mouse)

CAT: 0247-OKDD00633-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00633-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Mannose-binding lectin (protein A) 1|mannose-binding lectin (protein C) 2
Gene Aliases
L-MBP; mannan-binding protein; mannose binding lectin (A) ; mannose binding lectin, liver (C) ; mannose binding lectin, serum (A) ; mannose-binding protein A; mannose-binding protein C; MB; MBL; MBL-; MBL-A; MBL-C; MBP; MBP-; MBP-A; MBP-C; RA-reactive factor P28A subunit; ra-reactive factor polysaccharide-binding component p28B; raRF p28B; RARF/P28A; S-MBP.
Gene ID
17194|17195
Accession Number
NP_034905.1
Reactivity
Mouse|Mus musculus
Target
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology MBL ELISA Kit (Mouse) (OKDD00633) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MBL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MBL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MBL captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates.
Detection Range
0.156-10ng/mL
Sensitivity
<0.064 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
25 kDa
Notes
Formerly GWB-KBBTE2
Specificity
This assay has high sensitivity and excellent specificity for detection of MBL. No significant cross-reactivity or interference between MBL and analogues was observed.
Protein Length
239
NCBI Gene Symbol
Mbl1|Mbl2
Protein Name
Mannose-binding protein C
Gene Name URL
Mbl1|Mbl2
Nucleotide Accession Number
NM_010775.2

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