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RELA ELISA Kit (Rat)

CAT: 0247-OKDD00611-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00611-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
RELA proto-oncogene, NF-kB subunit
Gene Aliases
NFkB; nos2; nuclear factor kappa B subunit p65; nuclear factor kappaB subunit p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A.
Gene ID
309165
Accession Number
NP_954888.1
Reactivity
Rat|Rattus norvegicus
Target
Component of the nf-kappa b complex; involved in transcriptional activation [rgd, feb 2006]
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology RELA ELISA Kit (Rat) (OKDD00611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for RELA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RELA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RELA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
<0.049 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Notes
Formerly GWB-KBBWS9
Specificity
This assay has high sensitivity and excellent specificity for detection of RELA. No significant cross-reactivity or interference between RELA and analogues was observed.
Protein Length
550
NCBI Gene Symbol
Rela
Protein Name
RELA proto-oncogene, NF-kB subunit
Gene Name URL
Rela
Nucleotide Accession Number
NM_199267.2

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