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Slit3 ELISA Kit (Human)

CAT: 0247-OKDD00532-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00532-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Slit3 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Slit3 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Slit guidance ligand 3
Gene Aliases
MEGF5; multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains protein 5; SLIL2; slit homolog 3; slit homolog 3 protein; SLIT1; slit2; Slit-3.
Gene ID
6586
Accession Number
NP_001258875.1
Reactivity
Homo sapiens|Human
Target
May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology Slit3 ELISA Kit (Human) (OKDD00532) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for Slit3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slit3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Slit3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.058 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
168 kDa
Notes
Formerly GWB-KBBIF7
Specificity
This assay has high sensitivity and excellent specificity for detection of Slit3. No significant cross-reactivity or interference between Slit3 and analogues was observed.
Protein Length
1523
NCBI Gene Symbol
SLIT3
Protein Name
Slit homolog 3 protein
Gene Name URL
SLIT3
Nucleotide Accession Number
NM_001271946.1

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