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RS ELISA Kit (Human)

CAT: 0247-OKDD00513-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00513-96WSize:96 Tests
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level RS were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level RS were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Retinoschisin 1
Gene Aliases
Retinoschisin; RS; X-linked juvenile retinoschisis protein; XLRS1.
Gene ID
6247
Accession Number
NP_000321.1
Reactivity
Homo sapiens|Human
Target
Binds negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides (By similarity) . May play a role in cell-cell adhesion processes in the retina, via homomeric interaction between octamers present on the surface of two neighboring cells (PubMed:27114531) . Required for normal structure and function of the retina (PubMed:19093009) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology RS ELISA Kit (Human) (OKDD00513) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for RS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RS is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RS captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.069 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Notes
Formerly GWB-KBBIY7
Specificity
This assay has high sensitivity and excellent specificity for detection of RS. No significant cross-reactivity or interference between RS and analogues was observed.
Protein Length
224
NCBI Gene Symbol
RS1
Protein Name
Retinoschisin
Gene Name URL
RS1
Nucleotide Accession Number
NM_000330.3

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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