MMP9 ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level MMP9 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level MMP9 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

• Gene Name:

  Matrix metallopeptidase 9

• Gene Aliases:

  CLG4B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) ; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) ; matrix metalloproteinase-9; MMP-9; type V collagenase.

• Gene ID:

  4318

• Accession Number:

  NP_004985.2

• Reactivity:

  Homo sapiens|Human

• Target:

  Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked immunosorbent assay

• Assay Principle:

  Aviva Systems Biology MMP9 ELISA Kit (Human) (OKDD00397) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP9 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP9 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.063 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  78 kDa

• Notes:

  Formerly GWB-KBBWC1

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of MMP9. No significant cross-reactivity or interference between MMP9 and analogues was observed.

• Protein Length:

  707

• NCBI Gene Symbol:

  MMP9

• Protein Name:

  Matrix metalloproteinase-9

• Gene Name URL:

  MMP9

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_004994.2