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LY96 ELISA Kit (Human)

CAT: 0247-OKDD00383-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00383-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level LY96 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level LY96 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Lymphocyte antigen 96
Gene Aliases
ESOP-1; ly-96; lymphocyte antigen 96; MD2; MD-2; myeloid differentiation protein-2; protein MD-2.
Gene ID
23643
Accession Number
NP_001182726.1
Reactivity
Homo sapiens|Human
Target
Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869) . Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030) . Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581) . Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology LY96 ELISA Kit (Human) (OKDD00383) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LY96 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LY96 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LY96 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.06 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Notes
Formerly GWB-KBBNE3
Specificity
This assay has high sensitivity and excellent specificity for detection of LY96. No significant cross-reactivity or interference between LY96 and analogues was observed.
Protein Length
160
NCBI Gene Symbol
LY96
Protein Name
Lymphocyte antigen 96
Gene Name URL
LY96
Nucleotide Accession Number
NM_001195797.1

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