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LOR ELISA Kit (Human)

CAT: 0247-OKDD00380-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00380-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level LOR were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level LOR were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Loricrin cornified envelope precursor protein
Gene Aliases
LOR; loricrin.
Gene ID
4014
Accession Number
NP_000418.2
Reactivity
Homo sapiens|Human
Target
Major keratinocyte cell envelope protein.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology LOR ELISA Kit (Human) (OKDD00380) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LOR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LOR is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LOR captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Notes
Formerly GWB-KBBNR5
Specificity
This assay has high sensitivity and excellent specificity for detection of LOR. No significant cross-reactivity or interference between LOR and analogues was observed.
Protein Length
312
NCBI Gene Symbol
LORICRIN
Protein Name
Loricrin
Gene Name URL
LORICRIN
Nucleotide Accession Number
NM_000427.2

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