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GPX4 ELISA Kit (Human)

CAT: 0247-OKDD00295-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00295-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level GPX4 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level GPX4 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Glutathione peroxidase 4
Gene Aliases
Epididymis secretory sperm binding protein; Glutathione peroxidase 4; GPx-4; GSHPx-4; MCSP; PHGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; SMDS; snGPx; snPHGPx; sperm nucleus glutathione peroxidase.
Gene ID
2879
Accession Number
NP_001034936.1
Reactivity
Homo sapiens|Human
Target
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (By similarity) . Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity) . Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (By similarity) . Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (PubMed:24439385) . The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity) . The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity) . May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity) . Required for normal sperm development and male fertility (By similarity) . Essential for maturation and survival of photoreceptor cells (By similarity) . Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology GPX4 ELISA Kit (Human) (OKDD00295) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for GPX4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPX4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GPX4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture aupernartes
Detection Range
0.156-10ng/mL
Sensitivity
< 0.061 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Notes
Formerly GWB-KBBYP9
Specificity
This assay has high sensitivity and excellent specificity for detection of GPX4. No significant cross-reactivity or interference between GPX4 and analogues was observed.
Protein Length
197
NCBI Gene Symbol
GPX4
Protein Name
Phospholipid hydroperoxide glutathione peroxidase
Gene Name URL
GPX4
Nucleotide Accession Number
NM_001039847.2

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