EFNB2 ELISA Kit (Human)

CAT: 0247-OKDD00250-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKDD00250-96WSize:96 Tests
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level EFNB2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level EFNB2 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Ephrin B2
Gene Aliases
Eph-related receptor tyrosine kinase ligand 5; ephrin-B2; EPLG5; HTK ligand; Htk-L; HTKL; LERK5; ligand of eph-related kinase 5.
Gene ID
1948
Accession Number
NP_004084.1
Reactivity
Homo sapiens|Human
Target
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology EFNB2 ELISA Kit (Human) (OKDD00250) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for EFNB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EFNB2 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EFNB2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.053 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Notes
Formerly GWB-KBBDF8
Specificity
This assay has high sensitivity and excellent specificity for detection of EFNB2. No significant cross-reactivity or interference between EFNB2 and analogues was observed.
Protein Length
333
NCBI Gene Symbol
EFNB2
Protein Name
Ephrin-B2
Gene Name URL
EFNB2
Nucleotide Accession Number
NM_004093.3

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