DMKN ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level DMKN were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level DMKN were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

• Gene Name:

  Dermokine

• Gene Aliases:

  Dermokine; epidermis-specific secreted protein SK30/SK89; UNQ729; ZD52F10.

• Gene ID:

  93099

• Accession Number:

  NP_001119528.2

• Reactivity:

  Homo sapiens|Human

• Target:

  May act as a soluble regulator of keratinocyte differentiation.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked immunosorbent assay

• Assay Principle:

  Aviva Systems Biology DMKN ELISA Kit (Human) (OKDD00232) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for DMKN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DMKN is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DMKN captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates, Cell lysates

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.061 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  47 kDa

• Notes:

  Formerly GWB-KBBQT4

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of DMKN. No significant cross-reactivity or interference between DMKN and analogues was observed.

• Protein Length:

  476

• NCBI Gene Symbol:

  DMKN

• Protein Name:

  Dermokine

• Gene Name URL:

  DMKN

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001126056.2