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DHODH ELISA Kit (Human)

CAT: 0247-OKDD00228-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00228-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level DHODH were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level DHODH were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Dihydroorotate dehydrogenase (quinone)
Gene Aliases
DHOdehase; dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate oxidase; human complement of yeast URA1; POADS; URA1.
Gene ID
1723
Accession Number
NP_001352.2
Reactivity
Homo sapiens|Human
Target
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology DHODH ELISA Kit (Human) (OKDD00228) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for DHODH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DHODH is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DHODH captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.051 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Notes
Formerly GWB-KBBLF7
Specificity
This assay has high sensitivity and excellent specificity for detection of DHODH. No significant cross-reactivity or interference between DHODH and analogues was observed.
Protein Length
395
NCBI Gene Symbol
DHODH
Protein Name
Dihydroorotate dehydrogenase (quinone), mitochondrial
Gene Name URL
DHODH
Nucleotide Accession Number
NM_001361.4

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