CAP2 ELISA Kit (Human)

CAT: 0247-OKDD00164-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKDD00164-96WSize:96 Tests
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level CAP2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level CAP2 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Cyclase associated actin cytoskeleton regulatory protein 2
Gene Aliases
2810452G09Rik; adenylyl cyclase-associated protein 2; CAP 2; CAP, adenylate cyclase-associated protein, 2.
Gene ID
10486
Accession Number
NP_006357.1
Reactivity
Homo sapiens|Human
Target
May have a regulatory bifunctional role.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology CAP2 ELISA Kit (Human) (OKDD00164) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CAP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAP2 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CAP2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
53 kDa
Notes
Formerly GWB-KBBLB5
Specificity
This assay has high sensitivity and excellent specificity for detection of CAP2. No significant cross-reactivity or interference between CAP2 and analogues was observed.
Protein Length
477
NCBI Gene Symbol
CAP2
Protein Name
Adenylyl cyclase-associated protein 2
Gene Name URL
CAP2
Nucleotide Accession Number
NM_006366.2

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