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AGL ELISA Kit (Human)

CAT: 0247-OKCD09589-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD09589-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Glycogen Debranching Enzyme (AGL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Glycogen Debranching Enzyme (AGL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Aliases
Amylo-1, 6-glucosidase, 4-alpha-glucanotransferase; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided; GDE; glycogen debrancher; glycogen debranching enzyme; glycogen debranching protein.
Gene ID
178
Accession Number
NP_000019.2
Reactivity
Homo sapiens|Human
Target
Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Debranching Enzyme (AGL) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Debranching Enzyme (AGL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Debranching Enzyme (AGL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycogen Debranching Enzyme (AGL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) _x005F Serum (n=5) 94-105 99 _x005F EDTA plasma (n=5) 81-97 92 _x005F Heparin plasma (n=5) 79-93 86 _x005F Matrix Recovery Range (%) Average (%) _x005F Serum (n=5) 94-105 99 _x005F EDTA plasma (n=5) 81-97 92 _x005F Heparin plasma (n=5) 79-93 86 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 94-105 99 Serum (n=5) 94-105 99 EDTA plasma (n=5) 81-97 92 EDTA plasma (n=5) 81-97 92 Heparin plasma (n=5) 79-93 86 Heparin plasma (n=5) 79-93 86
Sensitivity
< 0.057 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-AGL Microplate 96 Wells (12 x 8 Well Strips) _x005F AGL Lyophilized Standard 2 x 50 ng _x005F 100X Biotinylated AGL Detector Antibody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Sample Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount _x005F Anti-AGL Microplate 96 Wells (12 x 8 Well Strips) _x005F AGL Lyophilized Standard 2 x 50 ng _x005F 100X Biotinylated AGL Detector Antibody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Sample Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-AGL Microplate 96 Wells (12 x 8 Well Strips) Anti-AGL Microplate 96 Wells (12 x 8 Well Strips) AGL Lyophilized Standard 2 x 50 ng AGL Lyophilized Standard 2 x 50 ng 100X Biotinylated AGL Detector Antibody 1 x 120 uL 100X Biotinylated AGL Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Sample Diluent 1 x 20 mL Sample Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Glycogen Debranching Enzyme (AGL) ._x005F No significant cross-reactivity or interference between Glycogen Debranching Enzyme (AGL) and analogues was observed.
Protein Length
1532
NCBI Gene Symbol
AGL
Protein Name
Glycogen debranching enzyme
Gene Name URL
AGL
Nucleotide Accession Number
NM_000028.2

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