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CUZD1 ELISA Kit (Mouse)

CAT: 0247-OKCD09464-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD09464-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
CUB and zona pellucida-like domains 1
Gene Aliases
CUB and zona pellucida-like domain-containing protein 1; CUB and ZP domain-containing protein 1; cuzd1; ERG-; Erg-1; integral membrane-associated protein 1; Itm; Itmap1; UO-4; Uo-44; Usg; UTCZ; Utczp.
Gene ID
16433
Reactivity
Mouse|Mus musculus
Target
CUZD1 antiserum inhibits cell attachment and proliferation of ovarian cancer cells so may be involved in these processes (By similarity) . May also play a role in the uterus during late pregnancy and/or in trypsin activation in pancreatic acinar cells.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CUB And Zona Pellucida Like Domains Protein 1 (CUZD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.068ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount CUZD1 Microplate 96 Wells (12 x 8 Well strips) CUZD1 Lyophilized Standard 2 100X CUZD1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount CUZD1 Microplate 96 Wells (12 x 8 Well strips) CUZD1 Microplate 96 Wells (12 x 8 Well strips) CUZD1 Lyophilized Standard 2 CUZD1 Lyophilized Standard 2 100X CUZD1 HRP-Detector Anitbody 1 x 120 uL 100X CUZD1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Cuzd1
Protein Name
CUB and zona pellucida-like domain-containing protein 1
Gene Name URL
Cuzd1

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