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CHAF1B ELISA Kit (Mouse)

CAT: 0247-OKCD09198-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD09198-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chromatin Assembly Factor 1, Subunit B (CHAF1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chromatin Assembly Factor 1, Subunit B (CHAF1B) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Chromatin assembly factor 1, subunit B (p60)
Gene Aliases
2600017H24Rik; C76145; CAF; CAF1; CAF-1 subunit B; CAF1A; CAF1P; CAF1P60; CAF-I; CAF-I 60 kDa subunit; CAF-I p60; CAF-Ip60; chromatin assembly factor 1 subunit B; chromatin assembly factor I p60 subunit; MPHO; MPHOSPH7.
Gene ID
110749
Accession Number
NP_082359.1
Reactivity
Mouse|Mus musculus
Target
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromatin Assembly Factor 1, Subunit B (CHAF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromatin Assembly Factor 1, Subunit B (CHAF1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.052ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount CHAF1B Microplate 96 Wells (12 x 8 Well strips) CHAF1B Lyophilized Standard 2 100X CHAF1B HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount CHAF1B Microplate 96 Wells (12 x 8 Well strips) CHAF1B Microplate 96 Wells (12 x 8 Well strips) CHAF1B Lyophilized Standard 2 CHAF1B Lyophilized Standard 2 100X CHAF1B HRP-Detector Anitbody 1 x 120 uL 100X CHAF1B HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Chaf1b
Protein Name
Chromatin assembly factor 1 subunit B
Gene Name URL
Chaf1b
Nucleotide Accession Number
NM_028083.4

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