Products for Research Use Only
RegisterLogin

IFITM2 ELISA Kit (Mouse)

CAT: 0247-OKCD09106-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD09106-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interferon Induced Transmembrane Protein 2 (IFITM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interferon Induced Transmembrane Protein 2 (IFITM2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Interferon induced transmembrane protein 2
Gene Aliases
Dispanin subfamily A member 2c; DSPA2c; fra; fragilis protein 3; fragilis R; fragilis3; Ifit; Ifitm3l; interferon induced transmembrane protein, like; interferon-induced transmembrane protein 2; mil; mil-3.
Gene ID
80876
Accession Number
NP_109619.1
Reactivity
Mouse|Mus musculus
Target
IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV) and West Nile virus (WNV) . Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Induced Transmembrane Protein 2 (IFITM2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Induced Transmembrane Protein 2 (IFITM2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Induced Transmembrane Protein 2 (IFITM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Induced Transmembrane Protein 2 (IFITM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.121ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount IFITM2 Microplate 96 Wells (12 x 8 Well strips) IFITM2 Lyophilized Standard 2 100X IFITM2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount IFITM2 Microplate 96 Wells (12 x 8 Well strips) IFITM2 Microplate 96 Wells (12 x 8 Well strips) IFITM2 Lyophilized Standard 2 IFITM2 Lyophilized Standard 2 100X IFITM2 HRP-Detector Anitbody 1 x 120 uL 100X IFITM2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Ifitm2
Protein Name
Interferon-induced transmembrane protein 2
Gene Name URL
Ifitm2
Nucleotide Accession Number
NM_030694.1

Related Products

CatalogName
OACA03134-100UGIFITM2 Antibody
OACA03134-50UGIFITM2 Antibody
OCOA01766-20UGIFITM2 Protein Lysate
ARP84933_P050-25ULIFITM2 Antibody - middle region
OASG03706-100ULIFITM2 Antibody - middle region
ARP84934_P050-25ULIFITM2 Antibody - middle region
OKEH05020-96WIFITM10 ELISA Kit (Mouse)
ARP84934_P050IFITM2 Antibody - middle region (ARP84934_P050)
ARP84933_P050IFITM2 Antibody - middle region (ARP84933_P050)
ARP46724_P050-25ULIFITM2 Antibody - N-terminal region

Alternative Products

CatalogName
E057027Mouse IFITM2 ELISA Kit
E3494Interferon-induced transmembrane protein 2 (IFITM2) Mouse ELISA Kit
RK02913Mouse Interferon Induced Transmembrane Protein 2 ELISA Kit (IFITM2)
GTR18813355-01Mouse Interferon Induced Transmembrane Protein 2 (IFITM2) ELISA Kit
EK247231Mouse IFITM2 (Interferon Induced Transmembrane Protein 2) ELISA Kit
KLM5768GENLISA™ Mouse Interferon Induced Transmembrane Protein 2 (IFITM2) ELISA
MBS9342088-01Mouse Interferon-induced transmembrane protein 2, IFITM2 ELISA Kit
517348Interferon Induced Transmembrane Protein 2 (IFITM2) BioAssayTM ELISA Kit (Mouse)
EK6F647-01Mouse Interferon Induced Transmembrane Protein 2 (IFITM2) ELISA Kit-Sandwich
157830-01Interferon Induced Transmembrane Protein 2 (IFITM2) BioAssayTM ECL ELISA Kit (Mouse) discontinued

Popular Products