MTFMT ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Mitochondrial methionyl-tRNA formyltransferase

• Gene Aliases:

  2310020P08Rik; methionyl-tRNA formyltransferase, mitochondrial.

• Gene ID:

  69606

• Accession Number:

  NP_081410.2

• Reactivity:

  Mouse|Mus musculus

• Target:

  Formylates methionyl-tRNA in mitochondria. A single tRNA (Met) gene gives rise to both an initiator and an elongator species via an unknown mechanism (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Methionyl tRNA Formyltransferase (MTFMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids.

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.055ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount MTFMT Microplate 96 Wells (12 x 8 Well strips) MTFMT Lyophilized Standard 2 100X MTFMT HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount MTFMT Microplate 96 Wells (12 x 8 Well strips) MTFMT Microplate 96 Wells (12 x 8 Well strips) MTFMT Lyophilized Standard 2 MTFMT Lyophilized Standard 2 100X MTFMT HRP-Detector Anitbody 1 x 120 uL 100X MTFMT HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• NCBI Gene Symbol:

  Mtfmt

• Protein Name:

  Methionyl-tRNA formyltransferase, mitochondrial

• Gene Name URL:

  Mtfmt

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_027134.3