Products for Research Use Only
RegisterLogin

SPTLC3 ELISA Kit (Mouse)

CAT: 0247-OKCD09028-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD09028-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Serine palmitoyltransferase, long chain base subunit 3
Gene Aliases
C130053K05Rik; LCB 3; LCB2b; long chain base biosynthesis protein 2b; long chain base biosynthesis protein 3; serine palmitoyltransferase 3; serine-palmitoyl-CoA transferase 3; serine-palmityl-CoA transferase 3; SPT 3.
Gene ID
228677
Accession Number
NP_780676.1
Reactivity
Mouse|Mus musculus
Target
Serine palmitoyltransferase (SPT) . The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.058ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount SPTLC3 Microplate 96 Wells (12 x 8 Well strips) SPTLC3 Lyophilized Standard 2 100X SPTLC3 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount SPTLC3 Microplate 96 Wells (12 x 8 Well strips) SPTLC3 Microplate 96 Wells (12 x 8 Well strips) SPTLC3 Lyophilized Standard 2 SPTLC3 Lyophilized Standard 2 100X SPTLC3 HRP-Detector Anitbody 1 x 120 uL 100X SPTLC3 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Sptlc3
Protein Name
Serine palmitoyltransferase 3
Gene Name URL
Sptlc3
Nucleotide Accession Number
NM_175467.3

Related Products

CatalogName
OKCD00522-96WSPTLC3 ELISA Kit (Human)
OKDD00542SPTLC3 ELISA Kit (Human) (OKDD00542)
OKAN06180SPTLC3 ELISA Kit (Human) (OKAN06180)
OKCD00522SPTLC3 ELISA Kit (Human) (OKCD00522)
OACD00956-100ULSPTLC3 Antibody
OACD00957-1MLSPTLC3 Antibody
OACA06025-50UGSPTLC3 Antibody
OACD00957-100ULSPTLC3 Antibody
OACD00956-1MLSPTLC3 Antibody
OACA06025-100UGSPTLC3 Antibody

Alternative Products

CatalogName
GTR18827138-01Mouse SPTLC3 ELISA Kit
GTR18833758-01Mouse SPTLC3 ELISA Kit (Ready to Use)
MBS9337931-01Mouse Serine palmitoyltransferase 3, SPTLC3 ELISA Kit
G9699Serine palmitoyltransferase 3 (SPTLC3) Mouse ELISA Kit
KTE70252-01Mouse Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit
MBS2706322-01Mouse Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) ELISA Kit
JL17488-01Mouse Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) ELISA Kit
EM23075Mouse SPTLC3 (Serine Palmitoyltransferase, Long Chain Base Subunit 3) ELISA Kit
G9703Serine Palmitoyltransferase (Long Chain Base Subunit 3 (SPTLC3) Mouse ELISA Kit
MBS8228551-01SPTLC3 siRNA (Mouse)

Popular Products