RegisterLogin

CRYBB2 ELISA Kit (Human)

CAT: 247-OKCD08776-96WSize: 96 WellDry Ice: NoHazardous: No
96 WellSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Crystallin Beta B2 (CRYbB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Crystallin Beta B2 (CRYbB2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Crystallin beta B2
Gene Aliases
Beta-B2 crystallin; beta-crystallin B2; beta-crystallin Bp; CCA2; CRYB2; CRYB2A; CTA-221G9.7; CTRCT3; D22S665; eye lens structural protein.
Gene ID
1415
Accession Number
NP_000487.1
Reactivity
Homo sapiens|Human
Target
Crystallins are the dominant structural components of the vertebrate eye lens.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Beta B2 (CRYbB2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Beta B2 (CRYbB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Beta B2 (CRYbB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Crystallin Beta B2 (CRYbB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.118ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount CRYBB2 Microplate 96 Wells (12 x 8 Well strips) CRYBB2 Lyophilized Standard 2 100X CRYBB2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount CRYBB2 Microplate 96 Wells (12 x 8 Well strips) CRYBB2 Microplate 96 Wells (12 x 8 Well strips) CRYBB2 Lyophilized Standard 2 CRYBB2 Lyophilized Standard 2 100X CRYBB2 HRP-Detector Anitbody 1 x 120 uL 100X CRYBB2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
CRYBB2
Protein Name
Beta-crystallin B2
Gene Name URL
CRYBB2
Nucleotide Accession Number
NM_000496.2

Related Products

CatalogName
OKCD08777-96WCRYBB2 ELISA Kit (Rat)
OKCD00968-96WCRYBB2 ELISA Kit (Mouse)
OKCD08777CRYBB2 ELISA Kit (Rat) (OKCD08777)
OKCD00968CRYBB2 ELISA Kit (Mouse) (OKCD00968)
OAAJ07872-100ULCRYBB2 Antibody
OPCD02444-10UGCRYBB2 Recombinant Protein
OPCD02444-200UGCRYBB2 Recombinant Protein
OPCD02444-50UGCRYBB2 Recombinant Protein
OCOA17372-20UGCRYBB2 Protein Lysate
OAAB18755-400ULCRYBB2 Antibody - N-terminal region

Alternative Products

CatalogName
EF008862Human CRYBB2 ELISA KIT
E004678Human CRYbB2 ELISA Kit
521866-01CRYBB2
MBS8200040-01CRYBB2 siRNA (Human)
MBS7617097-01Recombinant Human CRYBB2
abx901277-01Human CRYBB2 siRNA
P9418-01Recombinant Human CRYBB2
16879051CRYBB2 Adenovirus (Human)
E045688Mouse CRYbB2 ELISA Kit
E080069Rat CRYbB2 ELISA Kit

Popular Products