EEF1A1 ELISA Kit (Human)

CAT: 0247-OKCD08740-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD08740-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Eukaryotic translation elongation factor 1 alpha 1
Gene Aliases
CCS3; CCS-3; cervical cancer suppressor 3; CTCL tumor antigen; EE1A1; EEF-1; EEF1A; eEF1A-1; EF1A; EF1a-like protein; EF-1-alpha-1; EF-Tu; elongation factor 1 alpha subunit; elongation factor 1-alpha 1; elongation factor Tu; epididymis secretory sperm binding protein; eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; LENG7; leukocyte receptor cluster (LRC) member 7; leukocyte receptor cluster member 7; prostate tumor-inducing protein 1; PTI1; translation elongation factor 1 alpha 1-like 14.
Gene ID
1915
Accession Number
NP_001393.1
Reactivity
Homo sapiens|Human
Target
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.055ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount EEF1A1 Microplate 96 Wells (12 x 8 Well strips) EEF1A1 Lyophilized Standard 2 100X EEF1A1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount EEF1A1 Microplate 96 Wells (12 x 8 Well strips) EEF1A1 Microplate 96 Wells (12 x 8 Well strips) EEF1A1 Lyophilized Standard 2 EEF1A1 Lyophilized Standard 2 100X EEF1A1 HRP-Detector Anitbody 1 x 120 uL 100X EEF1A1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
EEF1A1
Protein Name
Elongation factor 1-alpha 1
Gene Name URL
EEF1A1
Nucleotide Accession Number
NM_001402.5

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