EFNA1 ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Ephrin A1 (EFNA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Ephrin A1 (EFNA1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Ephrin A1

• Gene Aliases:

  B61; ECKLG; EFL1; eph-related receptor tyrosine kinase ligand 1; ephrin-A1; epididymis secretory sperm binding protein; EPLG1; gastric cancer metastasis associated long noncoding RNA; GMAN; immediate early response protein B61; LERK1; LERK-1; ligand of eph-related kinase 1; TNF alpha-induced protein 4; TNFAIP4; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4.

• Gene ID:

  1942

• Accession Number:

  NP_004419.2

• Reactivity:

  Homo sapiens|Human

• Target:

  Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A1 (EFNA1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin A1 (EFNA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin A1 (EFNA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ephrin A1 (EFNA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.057ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount EFNA1 Microplate 96 Wells (12 x 8 Well strips) EFNA1 Lyophilized Standard 2 100X EFNA1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount EFNA1 Microplate 96 Wells (12 x 8 Well strips) EFNA1 Microplate 96 Wells (12 x 8 Well strips) EFNA1 Lyophilized Standard 2 EFNA1 Lyophilized Standard 2 100X EFNA1 HRP-Detector Anitbody 1 x 120 uL 100X EFNA1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL

• NCBI Gene Symbol:

  EFNA1

• Protein Name:

  Ephrin-A1

• Gene Name URL:

  EFNA1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_004428.2