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SPRR4 ELISA Kit (Human)

CAT: 0247-OKCD08541-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD08541-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Small Proline Rich Protein 4 (SPRR4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Small Proline Rich Protein 4 (SPRR4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Small proline rich protein 4
Gene Aliases
Small proline-rich protein 4.
Gene ID
163778
Accession Number
NP_775103.1
Reactivity
Homo sapiens|Human
Target
Cross-linked envelope protein of keratinocytes. Involved in UV-induced cornification.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Proline Rich Protein 4 (SPRR4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Proline Rich Protein 4 (SPRR4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Proline Rich Protein 4 (SPRR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Small Proline Rich Protein 4 (SPRR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.115ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount SPRR4 Microplate 96 Wells (12 x 8 Well strips) SPRR4 Lyophilized Standard 2 100X SPRR4 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount SPRR4 Microplate 96 Wells (12 x 8 Well strips) SPRR4 Microplate 96 Wells (12 x 8 Well strips) SPRR4 Lyophilized Standard 2 SPRR4 Lyophilized Standard 2 100X SPRR4 HRP-Detector Anitbody 1 x 120 uL 100X SPRR4 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
SPRR4
Protein Name
Small proline-rich protein 4
Gene Name URL
SPRR4
Nucleotide Accession Number
NM_173080.2

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