RegisterLogin

SYN2 ELISA Kit (Mouse)

CAT: 0247-OKCD08397-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD08397-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Synapsin II (SYN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Synapsin II (SYN2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Synapsin II
Gene Aliases
2900074L19Rik; AI836018; AI841723; Synapsin II; synapsin-2.
Gene ID
20965
Accession Number
NP_001104485.1
Reactivity
Mouse|Mus musculus
Target
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synapsin II (SYN2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synapsin II (SYN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synapsin II (SYN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Synapsin II (SYN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
< 30pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount SYN2 Microplate 96 Wells (12 x 8 Well strips) SYN2 Lyophilized Standard 2 100X SYN2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount SYN2 Microplate 96 Wells (12 x 8 Well strips) SYN2 Microplate 96 Wells (12 x 8 Well strips) SYN2 Lyophilized Standard 2 SYN2 Lyophilized Standard 2 100X SYN2 HRP-Detector Anitbody 1 x 120 uL 100X SYN2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Syn2
Protein Name
Synapsin-2
Gene Name URL
Syn2
Nucleotide Accession Number
NM_001111015.1

Related Products

CatalogName
OACD00692-100ULSYN2 Antibody
OACD00692-1MLSYN2 Antibody
OPCD00380-200UGSYN2 Recombinant Protein
OPCD00380-10UGSYN2 Recombinant Protein
OCOA06619-20UGSYN2 Protein Lysate
OPCD00380-50UGSYN2 Recombinant Protein
ARP79311_P050-25ULSYN2 Antibody - middle region
ARP79449_P050-25ULSYN2 Antibody - middle region
ARP79311_P050SYN2 Antibody - middle region (ARP79311_P050)
AAP79449-100UGSYN2 Peptide - middle region (AAP79449)

Alternative Products

CatalogName
E069284Mouse SYN2 ELISA Kit
MBS8806760-01Mouse SYN2 (Synapsin II) ELISA Kit
YP-W22682-01Mouse Synapsin II (SYN2) ELISA Kit
E1743Mo-01Mouse Synapsin-2, SYN2 ELISA KIT
abx585008-01Mouse Synapsin-2 (SYN2) ELISA Kit
JOT-EK1743MoMouse Synapsin-2,SYN2 ELISA KIT
H3887Synapsin-2 (SYN2) Mouse ELISA Kit
KLM1743GENLISA™ Mouse Synapsin II (SYN2) ELISA
RK03217Mouse Synapsin II ELISA Kit (SYN2)
ELK7789MS-01Mouse SYN2 (Synapsin II) Microsample ELISA Kit

Popular Products