MTTP ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Microsomal Triglyceride Transfer Protein (MTTP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Microsomal Triglyceride Transfer Protein (MTTP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Microsomal triglyceride transfer protein

• Gene Aliases:

  1810043K16Rik; microsomal triglyceride transfer protein B; microsomal triglyceride transfer protein large subunit; microsomal triglyceride transfer protein, large subunit; MT; MTP.

• Gene ID:

  17777

• Accession Number:

  NP_001156929.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. May be involved in regulating cholesteryl ester biosynthesis in cells that produce lipoproteins. Loads phospholipid into the C1D1 antigen-binding groove. Isoform 2 is critical for the development of natural killer T (NKT) cells. May have a role in the biogenesis of lipid droplets.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microsomal Triglyceride Transfer Protein (MTTP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microsomal Triglyceride Transfer Protein (MTTP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microsomal Triglyceride Transfer Protein (MTTP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Microsomal Triglyceride Transfer Protein (MTTP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.056ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount MTTP Microplate 96 Wells (12 x 8 Well strips) MTTP Lyophilized Standard 2 100X MTTP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount MTTP Microplate 96 Wells (12 x 8 Well strips) MTTP Microplate 96 Wells (12 x 8 Well strips) MTTP Lyophilized Standard 2 MTTP Lyophilized Standard 2 100X MTTP HRP-Detector Anitbody 1 x 120 uL 100X MTTP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• NCBI Gene Symbol:

  Mttp

• Protein Name:

  Microsomal triglyceride transfer protein large subunit

• Gene Name URL:

  Mttp

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001163457.1