Products for Research Use Only
RegisterLogin

LTBP1 ELISA Kit (Mouse)

CAT: 0247-OKCD08011-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD08011-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Latent transforming growth factor beta binding protein 1
Gene Aliases
9430031G15Rik;9830146M04; b2b1000C; b2b1000Clo; latent TGF beta binding protein; latent transforming growth factor beta binding protein 1L; latent-transforming growth factor beta-binding protein 1; Ltb; LTBP; Ltbp-1; Ltbp1L; Tgfb; TGF-beta1-BP-1; transforming growth factor beta-1-binding protein 1.
Gene ID
268977
Accession Number
NP_001318166.1
Reactivity
Mouse|Mus musculus
Target
May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extracellular matrix (ECM) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.053ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount LTBP1 Microplate 96 Wells (12 x 8 Well strips) LTBP1 Lyophilized Standard 2 100X LTBP1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount LTBP1 Microplate 96 Wells (12 x 8 Well strips) LTBP1 Microplate 96 Wells (12 x 8 Well strips) LTBP1 Lyophilized Standard 2 LTBP1 Lyophilized Standard 2 100X LTBP1 HRP-Detector Anitbody 1 x 120 uL 100X LTBP1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Ltbp1
Protein Name
Latent-transforming growth factor beta-binding protein 1
Gene Name URL
Ltbp1
Nucleotide Accession Number
NM_001331237.1

Related Products

CatalogName
OKCA01897LTBP1 ELISA Kit (Rat) (OKCA01897)
OKCA01897-96WOKCA01897-96W - LTBP1 ELISA Kit (Rat)
OACD05361-100ULLTBP1 Antibody
OACD05362-1MLLTBP1 Antibody
OACD05361-1MLLTBP1 Antibody
OACD05362-100ULLTBP1 Antibody
OPCD05050-10UGLTBP1 Recombinant Protein
OPCD05050-200UGLTBP1 Recombinant Protein
OPCD05049-200UGLTBP1 Recombinant Protein
OPCD05049-50UGLTBP1 Recombinant Protein

Alternative Products

CatalogName
E058366Mouse LTBP1 ELISA Kit
GTR18831849-01Mouse LTBP1 ELISA Kit (Ready to Use)
E6033Latent-transforming Growth Factor β-binding protein 1 (LTBP1) Mouse ELISA Kit
E6029Latent Transforming Growth Factor β Binding Protein 1 (LTBP1) Mouse ELISA Kit
MBS8806174-01Mouse LTBP1 (Latent Transforming Growth Factor Beta Binding Protein 1) ELISA Kit
RDR-LTBP1-Mu-96TestsMouse Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) ELISA Kit
EK10018Mouse Latent-transforming growth factor beta-binding protein 1 (LTBP1) ELISA Kit
JOT-EK1193Mo-01Mouse latent transforming growth factor beta binding protein 1,LTBP1 ELISA KIT
KLM1193GENLISA™ Mouse Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) ELISA
E1193Mo-01Mouse latent transforming growth factor beta binding protein 1, LTBP1 ELISA KIT

Popular Products