Products for Research Use Only
RegisterLogin

AMY1A ELISA Kit (Human)

CAT: 0247-OKCD07429-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD07429-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Salivary Alpha Amylase (AMY1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Salivary Alpha Amylase (AMY1A) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Amylase alpha 1A|amylase alpha 1B|amylase alpha 1C
Gene Aliases
1,4-alpha-D-glucan glucanohydrolase 1; alpha-amylase 1; alpha-amylase 1A; alpha-amylase 1B; alpha-amylase 1C; AMY1; amylase alpha 1A (salivary) ; amylase alpha 1B (salivary) ; amylase alpha 1C (salivary) ; amylase, salivary, alpha-1A; amylase, salivary, alpha-1B; amylase, salivary, alpha-1C; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A; salivary amylase alpha 1B; salivary amylase alpha 1C.
Gene ID
276|277|278
Accession Number
NP_001008219.1
Reactivity
Homo sapiens|Human
Target
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308) . Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Salivary Alpha Amylase (AMY1A) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Salivary Alpha Amylase (AMY1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Salivary Alpha Amylase (AMY1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Salivary Alpha Amylase (AMY1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, saliva and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 1.22ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount AMY1A Microplate 96 Wells (12 x 8 Well strips) AMY1A Lyophilized Standard 2 100X AMY1A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount AMY1A Microplate 96 Wells (12 x 8 Well strips) AMY1A Microplate 96 Wells (12 x 8 Well strips) AMY1A Lyophilized Standard 2 AMY1A Lyophilized Standard 2 100X AMY1A HRP-Detector Anitbody 1 x 120 uL 100X AMY1A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
AMY1A|AMY1B|AMY1C
Protein Name
Alpha-amylase 1|Alpha-amylase 1A|Alpha-amylase 1B|Alpha-amylase 1C
Gene Name URL
AMY1A|AMY1B|AMY1C
Nucleotide Accession Number
NM_001008218.1

Related Products

CatalogName
OACA06813-50ULAMY1A Antibody
OACA06813-100ULAMY1A Antibody
OCOA16648-20UGAMY1A Protein Lysate
OCOA13327-20UGAMY1A Protein Lysate
ARP54700_P050-25ULAmy1a Antibody - middle region
ARP97696_P050AMY1A Antibody Middle region
OPCD01281-10UGAMY1A more Recombinant Protein
ARP97696_P050-25ULAMY1A Antibody - middle region
OPCD01281-50UGAMY1A more Recombinant Protein
OPCD01281-200UGAMY1A more Recombinant Protein

Alternative Products

CatalogName
ASB-OKCD07429-96WAMY1A ELISA Kit (Human)
ELA-E1482hHuman AMY1A ELISA Kit
KBH20130GENLISA™ Human Salivary Alpha Amylase (AMY1A) ELISA
ELI-04866hHuman Alpha-amylase 1, AMY1A ELISA KIT
EKU02323Human Salivary Alpha Amylase (AMY1A) ELISA Kit
MBS9921798-01Human Amylase, Alpha 1A (AMY1A) ELISA Kit
EH24785Human AMY1A (Salivary Alpha Amylase) ELISA Kit
E0140HuHuman AMY1A/Alpha-amylase 1 ELISA Kit
abx575213-01Human Amylase Alpha 1, Salivary (AMY1A) ELISA Kit
abx378499-01Human Alpha-Amylase 1A (AMY1A) ELISA Development Kit

Popular Products