PPP3CA ELISA Kit (Mouse)

CAT: 0247-OKCD07318-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD07318-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Calcineurin (CaN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Calcineurin (CaN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Protein phosphatase 3, catalytic subunit, alpha isoform
Gene Aliases
2900074D19Rik; C; calcineurin A alpha; Calmodulin-dependent calcineurin A subunit alpha isoform; Caln; Calna; CAM-PRP catalytic subunit; CN; CnA; CNA alpha; PP2B alpha 1; PP2BA alpha; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform.
Gene ID
19055
Accession Number
NP_001280551.1
Reactivity
Mouse|Mus musculus
Target
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca (2+) -mediated signals (PubMed:7791792, PubMed:26794871) . Many of the substrates contain a PxIxIT motif and/or a LxVP motif (By similarity) . In response to increased Ca (2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (By similarity) . In response to increased Ca (2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (By similarity) . Dephosphorylates heat shock protein HSPB1 (By similarity) . Dephosphorylates and activates transcription factor NFATC1 (By similarity) . Dephosphorylates and inactivates transcription factor ELK1 (By similarity) . Dephosphorylates DARPP32 (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcineurin (CaN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcineurin (CaN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.054ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount PPP3CA Microplate 96 Wells (12 x 8 Well strips) PPP3CA Lyophilized Standard 2 100X PPP3CA HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount PPP3CA Microplate 96 Wells (12 x 8 Well strips) PPP3CA Microplate 96 Wells (12 x 8 Well strips) PPP3CA Lyophilized Standard 2 PPP3CA Lyophilized Standard 2 100X PPP3CA HRP-Detector Anitbody 1 x 120 uL 100X PPP3CA HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Ppp3ca
Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Gene Name URL
Ppp3ca
Nucleotide Accession Number
NM_001293622.1

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