CD69 ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level C-Type Lectin Domain Family 2, Member C (CLEC2C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level C-Type Lectin Domain Family 2, Member C (CLEC2C) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  CD69 molecule

• Gene Aliases:

  Activation inducer molecule; activation inducer molecule (AIM/CD69) ; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen) ; CLEC2C; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; early activation antigen CD69; early lymphocyte activation antigen; early T-cell activation antigen p60; GP32/28; leukocyte surface antigen Leu-23; MLR-3.

• Gene ID:

  969

• Accession Number:

  NP_001772.1

• Reactivity:

  Homo sapiens|Human

• Target:

  Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 2, Member C (CLEC2C) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 2, Member C (CLEC2C) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 2, Member C (CLEC2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Lectin Domain Family 2, Member C (CLEC2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  15.6-1,000pg/mL

• Sensitivity:

  < 6.1pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount CD69 Microplate 96 Wells (12 x 8 Well strips) CD69 Lyophilized Standard 2 100X CD69 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount CD69 Microplate 96 Wells (12 x 8 Well strips) CD69 Microplate 96 Wells (12 x 8 Well strips) CD69 Lyophilized Standard 2 CD69 Lyophilized Standard 2 100X CD69 HRP-Detector Anitbody 1 x 120 uL 100X CD69 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL

• NCBI Gene Symbol:

  CD69

• Protein Name:

  Early activation antigen CD69

• Gene Name URL:

  CD69

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001781.2