F13A1 ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Coagulation Factor XIII A1 Polypeptide (F13A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Coagulation Factor XIII A1 Polypeptide (F13A1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Coagulation factor XIII A chain

• Gene Aliases:

  BA525O21.1 (coagulation factor XIII, A1 polypeptide) ; coagulation factor XIII A chain; coagulation factor XIII, A polypeptide; coagulation factor XIII, A1 polypeptide; coagulation factor XIIIa; F13A; factor XIIIa; fibrin stabilizing factor, A subunit; fibrinoligase; FSF, A subunit; protein-glutamine gamma-glutamyltransferase A chain; TGase; transglutaminase A chain; transglutaminase. plasma.

• Gene ID:

  2162

• Accession Number:

  NP_000120.2

• Reactivity:

  Homo sapiens|Human

• Target:

  Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XIII A1 Polypeptide (F13A1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XIII A1 Polypeptide (F13A1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XIII A1 Polypeptide (F13A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coagulation Factor XIII A1 Polypeptide (F13A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Plasma

• Detection Range:

  0.188-12ng/mL

• Sensitivity:

  < 0.069ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount F13A1 Microplate 96 Wells (12 x 8 Well strips) F13A1 Lyophilized Standard 2 100X F13A1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount F13A1 Microplate 96 Wells (12 x 8 Well strips) F13A1 Microplate 96 Wells (12 x 8 Well strips) F13A1 Lyophilized Standard 2 F13A1 Lyophilized Standard 2 100X F13A1 HRP-Detector Anitbody 1 x 120 uL 100X F13A1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• NCBI Gene Symbol:

  F13A1

• Protein Name:

  Coagulation factor XIII A chain

• Gene Name URL:

  F13A1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_000129.3