ABCG2 ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level ATP Binding Cassette Transporter G2 (ABCG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level ATP Binding Cassette Transporter G2 (ABCG2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  ATP binding cassette subfamily G member 2 (Junior blood group)

• Gene Aliases:

  ABC15; ABCP; AI428558; ATP-binding cassette sub-family G member 2; ATP-binding cassette, sub-family G (WHITE), member 2; Bc; BCRP; Bcrp1; breast cancer resistance protein 1 homolog; broad substrate specificity ATP-binding cassette transporter ABCG2; mitoxantrone resistance protein 1; MXR; MXR1; urate exporter.

• Gene ID:

  26357

• Accession Number:

  NP_036050.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain (By similarity) . May play a role in early stem cell self-renewal by blocking differentiation.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter G2 (ABCG2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter G2 (ABCG2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter G2 (ABCG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter G2 (ABCG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates and other biological fluids.

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.055ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount ABCG2 Microplate 96 Wells (12 x 8 Well strips) ABCG2 Lyophilized Standard 2 100X ABCG2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount ABCG2 Microplate 96 Wells (12 x 8 Well strips) ABCG2 Microplate 96 Wells (12 x 8 Well strips) ABCG2 Lyophilized Standard 2 ABCG2 Lyophilized Standard 2 100X ABCG2 HRP-Detector Anitbody 1 x 120 uL 100X ABCG2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• NCBI Gene Symbol:

  Abcg2

• Protein Name:

  ATP-binding cassette sub-family G member 2

• Gene Name URL:

  Abcg2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_011920.3