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PTGS2 ELISA Kit (Mouse)

CAT: 0247-OKCD06690-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD06690-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cyclooxygenase-2 (COX 2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cyclooxygenase-2 (COX 2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Prostaglandin-endoperoxide synthase 2
Gene Aliases
COX; Cox-; Cox-2; COX2; cyclooxygenase-2; glucocorticoid-regulated inflammatory cyclooxygenase; gripghs; macrophage activation-associated marker protein P71/73; PES-2; Pgh; PGH synthase 2; PGHS; Pghs2; PGHS-2; PHS; PHS II; PHS-2; prostaglandin G/H synthase 2; prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; Tis; TIS10; TIS10 protein.
Gene ID
19225
Accession Number
NP_035328.2
Reactivity
Mouse|Mus musculus
Target
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclooxygenase-2 (COX 2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclooxygenase-2 (COX 2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclooxygenase-2 (COX 2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cyclooxygenase-2 (COX 2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
< 35pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount PTGS2 Microplate 96 Wells (12 x 8 Well strips) PTGS2 Lyophilized Standard 2 100X PTGS2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount PTGS2 Microplate 96 Wells (12 x 8 Well strips) PTGS2 Microplate 96 Wells (12 x 8 Well strips) PTGS2 Lyophilized Standard 2 PTGS2 Lyophilized Standard 2 100X PTGS2 HRP-Detector Anitbody 1 x 120 uL 100X PTGS2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Ptgs2
Protein Name
Prostaglandin G/H synthase 2
Gene Name URL
Ptgs2
Nucleotide Accession Number
NM_011198.4

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