Products for Research Use Only
RegisterLogin

ESR2 ELISA Kit (Human)

CAT: 0247-OKCD06344-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD06344-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Estrogen receptor 2
Gene Aliases
Erb; ER-BETA; ESRB; ESR-BETA; ESTRB; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor beta splice variant, ERbeta2delta7; estrogen receptor beta splice variant, ERbeta4delta7; estrogen receptor beta splice variant, ERbeta6; estrogen receptor beta splice variant, ERbeta6delta7; estrogen receptor beta splice variant, ERbeta7; estrogen receptor beta splice variant, ERbeta7delta7; estrogen receptor beta splice variant, ERbetaEx. 4L; estrogen receptor beta splice variant, ERbetaEx. 6L; NR3A2; nuclear receptor subfamily 3 group A member 2; ODG8; oestrogen receptor beta.
Gene ID
2100
Accession Number
NP_001035365.1
Reactivity
Homo sapiens|Human
Target
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560) . Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.127ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount ESR2 Microplate 96 Wells (12 x 8 Well strips) ESR2 Lyophilized Standard 2 100X ESR2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount ESR2 Microplate 96 Wells (12 x 8 Well strips) ESR2 Microplate 96 Wells (12 x 8 Well strips) ESR2 Lyophilized Standard 2 ESR2 Lyophilized Standard 2 100X ESR2 HRP-Detector Anitbody 1 x 120 uL 100X ESR2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
ESR2
Protein Name
Estrogen receptor beta
Gene Name URL
ESR2
Nucleotide Accession Number
NM_001040275.1

Related Products

CatalogName
OKCD00798-96WESR2 ELISA Kit (Mouse)
OKEH03501-96WESR2 ELISA Kit (Mouse)
OKEH08512-96WESR2 ELISA Kit (Bovine)
OKEH01633-96WESR2 ELISA Kit (Rat)
OKCD06345-96WESR2 ELISA Kit (Rat)
OKEH01632-96WESR2 ELISA Kit (Pig)
OKEH08513-96WESR2 ELISA Kit (Chicken)
OKCD03437-96WESR2 Chemi-Luminescent ELISA Kit (Human)
OKCD00798ESR2 ELISA Kit (Mouse) (OKCD00798)
OKEH03501ESR2 ELISA Kit (Mouse) (OKEH03501)

Alternative Products

CatalogName
ELA-E2300hHuman ESR2 ELISA Kit
abx151440-01Human Estrogen Receptor Beta (ESR2) ELISA Kit
ELI-07365hHuman Estrogen receptor beta, ESR2 ELISA KIT
E0823HuHuman ESR2/Estrogen receptor beta ELISA Kit
GTR19336951-01ESR2 siRNA (Human)
E052152Mouse ESR2 ELISA Kit
19499051ESR2 Adenovirus (Human)
abx901782-01Human ESR2 siRNA
P1025Recombinant human ESR2
E082563Bovine ESR2 ELISA Kit

Popular Products