MAP1A ELISA Kit (Rat)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Microtubule Associated Protein 1A (MAP1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Microtubule Associated Protein 1A (MAP1A) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• CAS Number :

  7732-18-5

• Gene Name :

  Microtubule-associated protein 1A

• Gene Aliases :

  MAP-1A; microtubule-associated protein 1A; Mtap1a.

• Gene ID :

  25152

• Accession Number :

  NP_112257.1

• Reactivity :

  Rat|Rattus norvegicus

• Target :

  Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1A (MAP1A) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1A (MAP1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1A (MAP1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Microtubule Associated Protein 1A (MAP1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  0.312-20ng/mL

• Sensitivity :

  < 0.128ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount MAP1A Microplate 96 Wells (12 x 8 Well strips) MAP1A Lyophilized Standard 2 100X MAP1A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount MAP1A Microplate 96 Wells (12 x 8 Well strips) MAP1A Microplate 96 Wells (12 x 8 Well strips) MAP1A Lyophilized Standard 2 MAP1A Lyophilized Standard 2 100X MAP1A HRP-Detector Anitbody 1 x 120 uL 100X MAP1A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL

• NCBI Gene Symbol :

  Map1a

• Protein Name :

  Microtubule-associated protein 1A

• Gene Name URL :

  Map1a

• Nucleotide Accession Number :

  NM_030995.1