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FGF13 ELISA Kit (Human)

CAT: 0247-OKCD05284-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD05284-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 13
Gene Aliases
DEE90; FGF-13; FGF2; FHF2; FHF-2; fibroblast growth factor 13; fibroblast growth factor homologous factor 2; LINC00889.
Gene ID
2258
Accession Number
NP_001132970.1
Reactivity
Homo sapiens|Human
Target
Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 13 (FGF13) and unlabeled High Sensitive Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.23-100pg/mL
Sensitivity
< 0.53pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount FGF13 Microplate 96 Wells (12 x 8 Well strips) FGF13 Lyophilized Standard 2 100X FGF13 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount FGF13 Microplate 96 Wells (12 x 8 Well strips) FGF13 Microplate 96 Wells (12 x 8 Well strips) FGF13 Lyophilized Standard 2 FGF13 Lyophilized Standard 2 100X FGF13 HRP-Detector Anitbody 1 x 120 uL 100X FGF13 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
FGF13
Protein Name
Fibroblast growth factor 13
Gene Name URL
FGF13
Nucleotide Accession Number
NM_001139498.1

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