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THSD7A ELISA Kit (Human)

CAT: 0247-OKCD04532-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04532-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Thrombospondin type 1 domain containing 7A
Gene Aliases
Thrombospondin type-1 domain-containing protein 7A; thrombospondin, type I, domain containing 7A.
Gene ID
221981
Accession Number
NP_056019.1
Reactivity
Homo sapiens|Human
Target
Thrombospondin type-1 domain-containing protein 7A: Plays a role in actin cytoskeleton rearrangement.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
< 1.22ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount THSD7A Microplate 96 Wells (12 x 8 Well strips) THSD7A Lyophilized Standard 2 100X THSD7A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount THSD7A Microplate 96 Wells (12 x 8 Well strips) THSD7A Microplate 96 Wells (12 x 8 Well strips) THSD7A Lyophilized Standard 2 THSD7A Lyophilized Standard 2 100X THSD7A HRP-Detector Anitbody 1 x 120 uL 100X THSD7A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
THSD7A
Protein Name
Thrombospondin type-1 domain-containing protein 7A
Gene Name URL
THSD7A
Nucleotide Accession Number
NM_015204.2

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