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VGF ELISA Kit (Mouse)

CAT: 0247-OKCD04458-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04458-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
VGF nerve growth factor inducible
Gene Aliases
Gm1052; neurosecretory protein VGF.
Gene ID
381677
Accession Number
NP_001034474.1
Reactivity
Mouse|Mus musculus
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF Nerve Growth Factor Inducible (VGF) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VGF Nerve Growth Factor Inducible (VGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF Nerve Growth Factor Inducible (VGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VGF Nerve Growth Factor Inducible (VGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95-103 98 EDTA Plasma (n=5) 79-98 85 Heparin Plasma (n=5) 97-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-103 98 Serum (n=5) 95-103 98 EDTA Plasma (n=5) 79-98 85 EDTA Plasma (n=5) 79-98 85 Heparin Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 97-105 101
Sensitivity
12.4 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Il19 Microplate 96 Wells (12 x 8 Well strips) Il19 Lyophilized Standard 2 100X Il19 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Il19 Microplate 96 Wells (12 x 8 Well strips) Il19 Microplate 96 Wells (12 x 8 Well strips) Il19 Lyophilized Standard 2 Il19 Lyophilized Standard 2 100X Il19 HRP-Detector Anitbody 1 x 120 uL 100X Il19 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Vgf
Protein Name
MCG18019
Gene Name URL
Vgf
Nucleotide Accession Number
NM_001039385.1

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