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PKM ELISA Kit (Mouse)

CAT: 0247-OKCD04443-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04443-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Immunoglobulin A (IgA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Immunoglobulin A (IgA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
Pk, Pk-, Pk3, Pk-2, Pk-3, Pkm2, AA414905, AL024370, AL024424
Gene ID
18746
Accession Number
NP_001240812
Reactivity
Mouse|Mus musculus
Target
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin A (IgA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin A (IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Immunoglobulin A (IgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 97-105 101 EDTA Plasma (n=5) 83-99 93 Heparin Plasma (n=5) 84-91 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97-105 101 Serum (n=5) 97-105 101 EDTA Plasma (n=5) 83-99 93 EDTA Plasma (n=5) 83-99 93 Heparin Plasma (n=5) 84-91 88 Heparin Plasma (n=5) 84-91 88
Sensitivity
0.069 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Pkm2 Microplate 96 Wells (12 x 8 Well strips) Pkm2 Lyophilized Standard 2 100X Pkm2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Pkm2 Microplate 96 Wells (12 x 8 Well strips) Pkm2 Microplate 96 Wells (12 x 8 Well strips) Pkm2 Lyophilized Standard 2 Pkm2 Lyophilized Standard 2 100X Pkm2 HRP-Detector Anitbody 1 x 120 uL 100X Pkm2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
PKM
Protein Name
Ig alpha chain C region
Gene Name URL
PKM
Nucleotide Accession Number
NM_001253883

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