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C2 ELISA Kit (Rat)

CAT: 0247-OKCD04396-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04396-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 2 (C2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 2 (C2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Complement C2
Gene Aliases
Complement C2; complement component 2.
Gene ID
24231
Accession Number
NP_757376.2
Reactivity
Rat|Rattus norvegicus
Target
Complement C2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 2 (C2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 2 (C2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 2 (C2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 2 (C2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.19-12ng/mL
Recovery
Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 98-105 101 _x005F EDTA Plasma (n=5) 80-104 85 _x005F Heparin Plasma (n=5) 84-93 89 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 Serum (n=5) 98-105 101 EDTA Plasma (n=5) 80-104 85 EDTA Plasma (n=5) 80-104 85 Heparin Plasma (n=5) 84-93 89 Heparin Plasma (n=5) 84-93 89
Sensitivity
< 0.08 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F C2 Microplate 96 Wells (12 x 8 Well strips) _x005F C2 Lyophilized Standard 2 _x005F 100X C2 HRP-Detector Anitbody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL Component Amount Component Amount C2 Microplate 96 Wells (12 x 8 Well strips) C2 Microplate 96 Wells (12 x 8 Well strips) C2 Lyophilized Standard 2 C2 Lyophilized Standard 2 100X C2 HRP-Detector Anitbody 1 x 120 uL 100X C2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
C2
Protein Name
Complement C2
Gene Name URL
C2
Nucleotide Accession Number
NM_172222.2

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