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CD83 ELISA Kit (Mouse)

CAT: 0247-OKCD04392-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04392-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cluster Of Differentiation 83 (CD83) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cluster Of Differentiation 83 (CD83) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
CD83 antigen
Gene Aliases
CD83 antigen; mCD83.
Gene ID
12522
Accession Number
NP_033986.1
Reactivity
Mouse|Mus musculus
Target
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 83 (CD83) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 83 (CD83) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 83 (CD83), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 83 (CD83) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
46.88-3,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 80-95 80 EDTA Plasma (n=5) 83-96 88 Heparin Plasma (n=5) 98-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 80-95 80 Serum (n=5) 80-95 80 EDTA Plasma (n=5) 83-96 88 EDTA Plasma (n=5) 83-96 88 Heparin Plasma (n=5) 98-105 101 Heparin Plasma (n=5) 98-105 101
Sensitivity
17.88 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Cd83 Microplate 96 Wells (12 x 8 Well strips) Cd83 Lyophilized Standard 2 100X Cd83 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Cd83 Microplate 96 Wells (12 x 8 Well strips) Cd83 Microplate 96 Wells (12 x 8 Well strips) Cd83 Lyophilized Standard 2 Cd83 Lyophilized Standard 2 100X Cd83 HRP-Detector Anitbody 1 x 120 uL 100X Cd83 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Cd83
Protein Name
CD83 antigen
Gene Name URL
Cd83
Nucleotide Accession Number
NM_009856.3

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