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HBG2 ELISA Kit (Human)

CAT: 0247-OKCD04384-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04384-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hemoglobin Gamma 2 (HBg2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hemoglobin Gamma 2 (HBg2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Hemoglobin subunit gamma 2
Gene Aliases
Abnormal hemoglobin; gamma globin; gamma-2-globin; gamma-globin chain; G-gamma globin Paulinia; hb F Ggamma; HBG-T1; hemoglobin gamma-2 chain; hemoglobin gamma-G chain; hemoglobin subunit gamma-2; hemoglobin, gamma G; methemoglobin; TNCY.
Gene ID
3048
Accession Number
NP_000175.1
Reactivity
Homo sapiens|Human
Target
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin Gamma 2 (HBg2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Gamma 2 (HBg2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin Gamma 2 (HBg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hemoglobin Gamma 2 (HBg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, erythrocyte lysates.
Detection Range
1.56-100ug/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 78-97 87 EDTA Plasma (n=5) 78-95 85 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-97 87 Serum (n=5) 78-97 87 EDTA Plasma (n=5) 78-95 85 EDTA Plasma (n=5) 78-95 85
Sensitivity
0.62 ug/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount HBg2 Microplate 96 Wells (12 x 8 Well strips) HBg2 Lyophilized Standard 2 100X HBg2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount HBg2 Microplate 96 Wells (12 x 8 Well strips) HBg2 Microplate 96 Wells (12 x 8 Well strips) HBg2 Lyophilized Standard 2 HBg2 Lyophilized Standard 2 100X HBg2 HRP-Detector Anitbody 1 x 120 uL 100X HBg2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
HBG2
Protein Name
Hemoglobin subunit gamma-2
Gene Name URL
HBG2
Nucleotide Accession Number
NM_000184.2

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