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PAMP ELISA Kit (Human)

CAT: 0247-OKCD04224-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04224-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Presenilin Associated Metalloprotease (PAMP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Presenilin Associated Metalloprotease (PAMP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
YME1 like 1 ATPase
Gene Aliases
ATP-dependent metalloprotease FtsH1; ATP-dependent metalloprotease FtsH1 homolog; ATP-dependent zinc metalloprotease YME1L1; FTSH; meg-4; MEG4; OPA11; PAMP; presenilin-associated metalloprotease; YME1L; YME1-like protein 1.
Gene ID
10730
Accession Number
NP_001240795.1
Reactivity
Homo sapiens|Human
Target
ATP-dependent metalloprotease that catalyzes the degradation of folded and unfolded proteins with a suitable degron sequence in the mitochondrial intermembrane region (PubMed:26923599, PubMed:27786171) . Plays an important role in regulating mitochondrial morphology and function by cleaving OPA1 at position S2, giving rise to a form of OPA1 that promotes maintenance of normal mitochondrial structure and mitochondrial protein metabolism (PubMed:18076378, PubMed:26923599, PubMed:27495975) . Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins (PubMed:22262461) . Required for normal, constitutive degradation of PRELID1 (PubMed:27495975) . Catalyzes the degradation of OMA1 in response to membrane depolarization (PubMed:26923599) . Required to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1) (PubMed:22262461) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Presenilin Associated Metalloprotease (PAMP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Presenilin Associated Metalloprotease (PAMP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Presenilin Associated Metalloprotease (PAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Presenilin Associated Metalloprotease (PAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.115 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount PAMP Microplate 96 Wells (12 x 8 Well strips) PAMP Lyophilized Standard 2 100X PAMP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount PAMP Microplate 96 Wells (12 x 8 Well strips) PAMP Microplate 96 Wells (12 x 8 Well strips) PAMP Lyophilized Standard 2 PAMP Lyophilized Standard 2 100X PAMP HRP-Detector Anitbody 1 x 120 uL 100X PAMP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
YME1L1
Protein Name
ATP-dependent zinc metalloprotease YME1L1
Gene Name URL
YME1L1
Nucleotide Accession Number
NM_001253866.1

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