Recombinant Mouse NT-proBNP Protein (Trx Tag)

• Background:

  Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from the ventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.

• Abbreviation:

  NT-proBNP

• Synonyms:

  B-Type Natriuretic Peptide; GC-B; Natriuretic Peptide Precursor B; NT-Pro-BNP; Nppb

• UniProt:

  P40753

• Accession Number:

  P40753

• Expression System:

  E. coli

• Tag:

  N-Trx

• Sequence:

  Ser26-Arg76

• Field of Research:

  Cardiovascular; Metabolism

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  25.5 kDa

• Observed Molecular Weight:

  30 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse