GDF5 Chemi-Luminescent ELISA Kit (Rat)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Growth differentiation factor 5

• Gene Aliases:

  Cartilage-derived morphogenetic protein 1; Cdmp1; growth differentiation factor 5; growth/differentiation factor 5.

• Gene ID:

  252835

• Accession Number:

  XP_001066344

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Growth differentiation factor 5

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 5 (GDF5) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 5 (GDF5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 5 (GDF5) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  13.7-10,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 87-102 94 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 99-105 102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 87-102 94 Serum (n=5) 87-102 94 EDTA Plasma (n=5) 95-103 99 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 99-105 102

• Sensitivity:

  0.156 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Gdf5 Microplate 96 Wells (12 x 8 Well strips) Gdf5 Lyophilized Standard 2 100X Gdf5 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Gdf5 Microplate 96 Wells (12 x 8 Well strips) Gdf5 Microplate 96 Wells (12 x 8 Well strips) Gdf5 Lyophilized Standard 2 Gdf5 Lyophilized Standard 2 100X Gdf5 Biotinylated Detector Anitbody 1 x 120 uL 100X Gdf5 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  Gdf5

• Protein Name:

  Growth differentiation factor 5

• Gene Name URL:

  Gdf5

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  XM_001066344