FGF4 Chemi-Luminescent ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Fibroblast growth factor 4

• Gene Aliases:

  Fgf-4; Fgf7a; Fgfk; fibroblast growth factor 4; fibroblast growth factor 7a; HBGF-4; heparin-binding growth factor 4; hst; hst-1; Hst1; Hstf-; Hstf-1; kFGF; K-fibroblast growth factor; KS3.

• Gene ID:

  14175

• Accession Number:

  NP_034332

• Reactivity:

  Mouse|Mus musculus

• Target:

  Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Is essential for survival of the postimplantation mouse embryo. Required for normal limb and cardiac valve development during embryogenesis.

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 4 (FGF4) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 4 (FGF4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 4 (FGF4) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  1.37-1,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 85-96 90 EDTA Plasma (n=5) 82-94 90 Heparin Plasma (n=5) 78-101 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 85-96 90 Serum (n=5) 85-96 90 EDTA Plasma (n=5) 82-94 90 EDTA Plasma (n=5) 82-94 90 Heparin Plasma (n=5) 78-101 92 Heparin Plasma (n=5) 78-101 92

• Sensitivity:

  15.63 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Fgf4 Microplate 96 Wells (12 x 8 Well strips) Fgf4 Lyophilized Standard 2 100X Fgf4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Fgf4 Microplate 96 Wells (12 x 8 Well strips) Fgf4 Microplate 96 Wells (12 x 8 Well strips) Fgf4 Lyophilized Standard 2 Fgf4 Lyophilized Standard 2 100X Fgf4 Biotinylated Detector Anitbody 1 x 120 uL 100X Fgf4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  Fgf4

• Protein Name:

  Fibroblast growth factor 4

• Gene Name URL:

  Fgf4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_010202