ADIPOQ Chemi-Luminescent ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Adiponectin (ADPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Adiponectin (ADPN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Adiponectin, C1Q and collagen domain containing

• Gene Aliases:

  30 kDa adipocyte complement-related protein;30kDa; a; Acd; Acdc; Acr; Acrp30; Ad; Adid; adip; adipo; adipocyte complement related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; adipocyte-specific protein AdipoQ; adiponectin; adiponectin d; ap; apM1; APN; GBP2; GBP28.

• Gene ID:

  11450

• Accession Number:

  NP_033735

• Reactivity:

  Mouse|Mus musculus

• Target:

  Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADPN) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Adiponectin (ADPN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Adiponectin (ADPN) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  5.49-4,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 80-96 92 EDTA Plasma (n=5) 80-92 87 Heparin Plasma (n=5) 87-95 91 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 80-96 92 Serum (n=5) 80-96 92 EDTA Plasma (n=5) 80-92 87 EDTA Plasma (n=5) 80-92 87 Heparin Plasma (n=5) 87-95 91 Heparin Plasma (n=5) 87-95 91

• Sensitivity:

  5.49 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Adipoq Microplate 96 Wells (12 x 8 Well strips) Adipoq Lyophilized Standard 2 100X Adipoq Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Adipoq Microplate 96 Wells (12 x 8 Well strips) Adipoq Microplate 96 Wells (12 x 8 Well strips) Adipoq Lyophilized Standard 2 Adipoq Lyophilized Standard 2 100X Adipoq Biotinylated Detector Anitbody 1 x 120 uL 100X Adipoq Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  Adipoq

• Protein Name:

  Adiponectin

• Gene Name URL:

  Adipoq

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009605