Products for Research Use Only
RegisterLogin

TOP2A ELISA Kit (Mouse)

CAT: 0247-OKCD02945-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD02945-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Topoisomerase (DNA) II alpha
Gene Aliases
DNA topoisomerase 2-alpha; DNA Topoisomerase II alpha; DNA topoisomerase II, alpha isozyme; Top; Top-2.
Gene ID
21973
Accession Number
NP_035753.2
Reactivity
Mouse|Mus musculus
Target
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (PubMed:24321095) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase II (TOP2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase II (TOP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase II (TOP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Topoisomerase II (TOP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.116 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-TOP2A Microplate 96 Wells (12 x 8 Well strips) TOP2A Lyophilized Standard 2 100X Biotinylated TOP2A Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-TOP2A Microplate 96 Wells (12 x 8 Well strips) Anti-TOP2A Microplate 96 Wells (12 x 8 Well strips) TOP2A Lyophilized Standard 2 TOP2A Lyophilized Standard 2 100X Biotinylated TOP2A Detector Antibody 1 x 120 uL 100X Biotinylated TOP2A Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Topoisomerase II (TOP2) . No significant cross-reactivity or interference between Topoisomerase II (TOP2) and analogues was observed.
Protein Length
1528
NCBI Gene Symbol
Top2a
Protein Name
DNA topoisomerase 2-alpha
Gene Name URL
Top2a
Nucleotide Accession Number
NM_011623.2

Related Products

CatalogName
OKCD06826-96WTOP2A ELISA Kit (Rat)
OKCD06825-96WTOP2A ELISA Kit (Human)
OKEH01846-96WTOP2A ELISA Kit (Human)
OKEH08686-96WTOP2A ELISA Kit (Chicken)
OKEH08686TOP2A ELISA Kit (Chicken) (OKEH08686)
OKAN04799TOP2A ELISA Kit (Human) (OKAN04799)
OKCD06826TOP2A ELISA Kit (Rat) (OKCD06826)
OKCD06825TOP2A ELISA Kit (Human) (OKCD06825)
OKEH01846TOP2A ELISA Kit (Human) : 96 Wells (OKEH01846)
OKAG01087TOP2A Colorimetric Cell-Based ELISA Kit (OKAG01087)

Alternative Products

CatalogName
E051131Mouse TOP2A ELISA Kit
C9503DNA topoisomerase 2-α (TOP2A) Mouse ELISA Kit
JOT-EK2713Mo-01Mouse DNA Topoisomerase 2 Alpha,Top2A ELISA KIT
MBS2804115-01Mouse DNA topoisomerase 2-alpha (TOP2A) ELISA Kit
MBS1608587-01Mouse DNA Topoisomerase 2 Alpha, Top2A ELISA Kit
ELI-17021mMouse DNA topoisomerase 2-alpha, Top2a ELISA KIT
E1515MoMouse Top2a/DNA topoisomerase 2-alpha ELISA Kit
abx520740Mouse Topoisomerase (DNA) II Alpha (TOP2A) ELISA Kit
GTR10377756Mouse DNA topoisomerase 2 Alpha (TOP2A) ELISA kit
NBP2-53282-20ugMouse Monoclonal TOP2A Antibody (TOP2A/1362)

Popular Products