TNFSF14 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Tumor necrosis factor (ligand) superfamily, member 14

HVEM; HVEML; HVEM-L; LIG; LIGHT; LTg; Ly113; Tnlg1d; tumor necrosis factor ligand 1d; tumor necrosis factor ligand superfamily member 14.

50930

NP_062291.1

Mouse|Mus musculus

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB and stimulates the proliferation of T-cells.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, tissue homogenates and other biological fluids

15.6-1,000pg/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 78-88 83 EDTA Plasma (n=5) 92-101 96 Heparin Plasma (n=5) 87-95 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-88 83 Serum (n=5) 78-88 83 EDTA Plasma (n=5) 92-101 96 EDTA Plasma (n=5) 92-101 96 Heparin Plasma (n=5) 87-95 92 Heparin Plasma (n=5) 87-95 92

6.3 pg/mL

2°C to 8°C|-20°C

Component Amount Anti-TNFSF14 Microplate 96 Wells (12 x 8 Well strips) TNFSF14 Lyophilized Standard 2 100X Biotinylated TNFSF14 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-TNFSF14 Microplate 96 Wells (12 x 8 Well strips) Anti-TNFSF14 Microplate 96 Wells (12 x 8 Well strips) TNFSF14 Lyophilized Standard 2 TNFSF14 Lyophilized Standard 2 100X Biotinylated TNFSF14 Detector Antibody 1 x 120 uL 100X Biotinylated TNFSF14 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) . No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) and analogues was observed.

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Tnfsf14

Tumor necrosis factor ligand superfamily member 14

Tnfsf14