TMPO ELISA Kit (Human)

CAT: 0247-OKCD02937-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02937-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Thymopoietin (TMPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Thymopoietin (TMPO) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Thymopoietin
Gene Aliases
CMD1T; lamina-associated polypeptide 2; LAP2; LEM domain containing 4; LEMD4; PRO0868; thymopoietin; Thymopoietin, isoforms beta/gamma; Thymopoietin-related peptide isoforms beta/gamma; TP.
Gene ID
7112
Accession Number
NP_001027454.1
Reactivity
Homo sapiens|Human
Target
May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymopoietin (TMPO) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymopoietin (TMPO) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymopoietin (TMPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thymopoietin (TMPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
7.8-500pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 97 EDTA Plasma (n=5) 80-95 91 Heparin Plasma (n=5) 96-103 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 97 Serum (n=5) 93-101 97 EDTA Plasma (n=5) 80-95 91 EDTA Plasma (n=5) 80-95 91 Heparin Plasma (n=5) 96-103 99 Heparin Plasma (n=5) 96-103 99
Sensitivity
12.9 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-TMPO Microplate 96 Wells (12 x 8 Well strips) TMPO Lyophilized Standard 2 100X Biotinylated TMPO Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-TMPO Microplate 96 Wells (12 x 8 Well strips) Anti-TMPO Microplate 96 Wells (12 x 8 Well strips) TMPO Lyophilized Standard 2 TMPO Lyophilized Standard 2 100X Biotinylated TMPO Detector Antibody 1 x 120 uL 100X Biotinylated TMPO Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Thymopoietin (TMPO) . No significant cross-reactivity or interference between Thymopoietin (TMPO) and analogues was observed.
Protein Length
454
NCBI Gene Symbol
TMPO
Protein Name
Lamina-associated polypeptide 2, isoforms beta/gamma
Gene Name URL
TMPO
Nucleotide Accession Number
NM_001032283.2

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