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TFF2 ELISA Kit (Mouse)

CAT: 0247-OKCD02923-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02923-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Trefoil Factor 2 (TFF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Trefoil Factor 2 (TFF2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Trefoil factor 2 (spasmolytic protein 1)
Gene Aliases
Spasmolytic polypeptide.
Accession Number
NP_033389.2
Reactivity
Mouse|Mus musculus
Target
Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 2 (TFF2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trefoil Factor 2 (TFF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trefoil Factor 2 (TFF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Trefoil Factor 2 (TFF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Detection Range
31.2-2,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 80-103 79 _x005F EDTA Plasma (n=5) 89-101 93 _x005F Heparin Plasma (n=5) 98-105 101 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 80-103 79 Serum (n=5) 80-103 79 EDTA Plasma (n=5) 89-101 93 EDTA Plasma (n=5) 89-101 93 Heparin Plasma (n=5) 98-105 101 Heparin Plasma (n=5) 98-105 101
Sensitivity
< 12.2 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-TFF2 Microplate 96 Wells (12 x 8 Well strips) _x005F TFF2 Lyophilized Standard 2 _x005F 100X Biotinylated TFF2 Detector Antibody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F Stop Solution 1 x 6 mL _x005F TMB Substrate 1 x 9 mL _x005F Component Amount Component Amount Anti-TFF2 Microplate 96 Wells (12 x 8 Well strips) Anti-TFF2 Microplate 96 Wells (12 x 8 Well strips) TFF2 Lyophilized Standard 2 TFF2 Lyophilized Standard 2 100X Biotinylated TFF2 Detector Antibody 1 x 120 uL 100X Biotinylated TFF2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 2 (TFF2) ._x005F No significant cross-reactivity or interference between Trefoil Factor 2 (TFF2) and analogues was observed.
Protein Length
129
NCBI Gene Symbol
TFF2
Protein Name
Trefoil factor 2
Gene Name URL
TFF2
Nucleotide Accession Number
NM_009363.3

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